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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has become observed in several tumors, which leads to chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants were described but in depth nucleotide sequence information will not be but out there.

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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily has the epitope recognized through the antibody.

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This guidebook outlines even further attainable optimization of cellular and immunoassay parameters to be certain the very best final results are...

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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.

Focus on Facts Cyclin E belongs on the very conserved cyclin loved ones, whose members are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E forms a posh with and features as a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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